Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models.
Identifieur interne : 003F71 ( Main/Exploration ); précédent : 003F70; suivant : 003F72Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models.
Auteurs : Qian Feng [République populaire de Chine] ; Yingying Liu ; Xiuxia Qu ; Hongkui Deng ; Mingxiao Ding ; Terence L T. Lau ; Albert Cheung-Hoi Yu ; Jianguo ChenSource :
- DNA and cell biology [ 1044-5498 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Antigènes de surface (immunologie), Antigènes de surface (métabolisme), Antigènes viraux (immunologie), Antigènes viraux (métabolisme), Baculoviridae (immunologie), Baculoviridae (métabolisme), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Organismes génétiquement modifiés, Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Souris, Souris de lignée BALB C, Spodoptera (virologie), Virus du SRAS (immunologie).
- MESH :
- immunologie : Antigènes de surface, Antigènes viraux, Baculoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- métabolisme : Antigènes de surface, Antigènes viraux, Baculoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- virologie : Spodoptera.
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Organismes génétiquement modifiés, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antigens, Surface (immunology), Antigens, Surface (metabolism), Antigens, Viral (immunology), Antigens, Viral (metabolism), Baculoviridae (immunology), Baculoviridae (metabolism), Female, Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Organisms, Genetically Modified, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Spodoptera (virology), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , immunology : Antigens, Surface, Antigens, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Antigens, Surface, Antigens, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : Baculoviridae, SARS Virus.
- metabolism : Baculoviridae.
- virology : Spodoptera.
- Animals, Female, Mice, Mice, Inbred BALB C, Organisms, Genetically Modified, Spike Glycoprotein, Coronavirus.
Abstract
The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.
DOI: 10.1089/dna.2006.25.668
PubMed: 17184168
Affiliations:
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Le document en format XML
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Lau</name></author><author><name sortKey="Yu, Albert Cheung Hoi" sort="Yu, Albert Cheung Hoi" uniqKey="Yu A" first="Albert Cheung-Hoi" last="Yu">Albert Cheung-Hoi Yu</name></author><author><name sortKey="Chen, Jianguo" sort="Chen, Jianguo" uniqKey="Chen J" first="Jianguo" last="Chen">Jianguo Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17184168</idno><idno type="pmid">17184168</idno><idno type="doi">10.1089/dna.2006.25.668</idno><idno type="wicri:Area/PubMed/Corpus">001F44</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F44</idno><idno type="wicri:Area/PubMed/Curation">001F44</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F44</idno><idno type="wicri:Area/PubMed/Checkpoint">002279</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002279</idno><idno type="wicri:Area/Ncbi/Merge">001816</idno><idno type="wicri:Area/Ncbi/Curation">001816</idno><idno type="wicri:Area/Ncbi/Checkpoint">001816</idno><idno type="wicri:doubleKey">1044-5498:2006:Feng Q:baculovirus:surface:display</idno><idno type="wicri:Area/Main/Merge">004180</idno><idno type="wicri:Area/Main/Curation">003F71</idno><idno type="wicri:Area/Main/Exploration">003F71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models.</title><author><name sortKey="Feng, Qian" sort="Feng, Qian" uniqKey="Feng Q" first="Qian" last="Feng">Qian Feng</name><affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName></affiliation></author><author><name sortKey="Liu, Yingying" sort="Liu, Yingying" uniqKey="Liu Y" first="Yingying" last="Liu">Yingying Liu</name></author><author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name></author><author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name></author><author><name sortKey="Lau, Terence L T" sort="Lau, Terence L T" uniqKey="Lau T" first="Terence L T" last="Lau">Terence L T. Lau</name></author><author><name sortKey="Yu, Albert Cheung Hoi" sort="Yu, Albert Cheung Hoi" uniqKey="Yu A" first="Albert Cheung-Hoi" last="Yu">Albert Cheung-Hoi Yu</name></author><author><name sortKey="Chen, Jianguo" sort="Chen, Jianguo" uniqKey="Chen J" first="Jianguo" last="Chen">Jianguo Chen</name></author></analytic><series><title level="j">DNA and cell biology</title><idno type="ISSN">1044-5498</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Surface (immunology)</term><term>Antigens, Surface (metabolism)</term><term>Antigens, Viral (immunology)</term><term>Antigens, Viral (metabolism)</term><term>Baculoviridae (immunology)</term><term>Baculoviridae (metabolism)</term><term>Female</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Organisms, Genetically Modified</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spodoptera (virology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes de surface (immunologie)</term><term>Antigènes de surface (métabolisme)</term><term>Antigènes viraux (immunologie)</term><term>Antigènes viraux (métabolisme)</term><term>Baculoviridae (immunologie)</term><term>Baculoviridae (métabolisme)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Organismes génétiquement modifiés</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spodoptera (virologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Surface</term><term>Antigens, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Surface</term><term>Antigens, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes de surface</term><term>Antigènes viraux</term><term>Baculoviridae</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Baculoviridae</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Baculoviridae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes de surface</term><term>Antigènes viraux</term><term>Baculoviridae</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Spodoptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Organisms, Genetically Modified</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Organismes génétiquement modifiés</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Jianguo" sort="Chen, Jianguo" uniqKey="Chen J" first="Jianguo" last="Chen">Jianguo Chen</name><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name><name sortKey="Lau, Terence L T" sort="Lau, Terence L T" uniqKey="Lau T" first="Terence L T" last="Lau">Terence L T. Lau</name><name sortKey="Liu, Yingying" sort="Liu, Yingying" uniqKey="Liu Y" first="Yingying" last="Liu">Yingying Liu</name><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><name sortKey="Yu, Albert Cheung Hoi" sort="Yu, Albert Cheung Hoi" uniqKey="Yu A" first="Albert Cheung-Hoi" last="Yu">Albert Cheung-Hoi Yu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Feng, Qian" sort="Feng, Qian" uniqKey="Feng Q" first="Qian" last="Feng">Qian Feng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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